Cell Assays, Pharmacol.
[Fluorescence and HTS
[Cell Assays, Pharmacol.] 
[Biosensors
[Immunochem., Liposomes
[Membrane Biophysics
[Chemical Physics of Water] 

 These publications involve microphysiometry, a biosensor-based technology that detects cellular responses to pharmacological and toxic agents by monitoring changes in the rate of cellular metabolism in real time.  

Owicki, J.C., Parce, J.W., Kercso, K.M., Sigal, G.B., Muir, V.C., Venter, J.C., Fraser, C.M., & McConnell, H.M. (1990) Continuous Monitoring of Receptor-Mediated Changes in the Metabolic Rates of Living Cells.  Proc. Natl. Acad. Sci. U.S.A. 87:4007-4011.

Wada, H.G., Owicki, J.C., and Parce, J.W. (1991) Cells on Silicon: Bioassays with a Microphysiometer. Clinical Chemistry 37: 600-601.

Bruner, L.H., Kercso, K.M., Owicki, J.C., Parce, J.W., & Muir, V.C. (1990) Testing Ocular Irritancy In Vitro with the Silicon Microphysiometer. Toxicology In Vitro 5:277-284.

Parce, J.W., Owicki, J.C., Wada, H.G., & Kercso, K.M. (1991) Cells on Silicon: The Microphysiometer.  pp. 97-106 In: Alternative Methods in Toxicology, Volume 8:  Mary Ann Liebert, Inc., New York, NY.

Owicki, J.C., & Parce, J.W. (1992) Biosensors Based on the Energy Metabolism of Living Cells: The Physical Chemistry and Cell Biology of Extracellular Acidification.  Biosensors and Bioelectronics, 7:255-272.

Raley-Susman, K.M., Miller, K.M., Owicki, J.C., & Sapolsky, R.M. (1992) Effects of Excitotoxin Exposure on Metabolic Rate of Primary Hippocampal Cultures:  Application of Silicon Microphysiometry to Neurobiology.  J. Neurosci. 12:773-780.

Wada, H.G., Owicki, J.C., Bruner, L.H., Miller, K.R., Raley-Susman, K.M., Panfili, P.R., Humphries, G.M.K., & Parce, J.W. (1992) Measurement of Cellular Responses to Toxic Agents using a Silicon Microphysiometer.  AATEX 1:154-164.

Baxter, G.T., Miller, D.L., Kuo, R.C., Wada, H.G., & Owicki, J.C. (1992) PKCe  is involved in GM-CSF Signal Transduction: Evidence from Microphysiometry and Antisense Oligonucleotide Experiments.  Biochemistry 31:10950-10954.

Miller, D.L., Olson, J.C., Parce, J.W., & Owicki, J.C. (1993) Cholinergic Stimulation of the Na+/K+ ATPase as Revealed by Microphysiometry.  Biophys. J. 64:813-823

Kuo, R.C., Baxter, G.T., Alajoki, L., Miller, D.L., Libby, J.M., & Owicki, J.C. (1993) A Metabolic View of Receptor Activation in Cultured Cells Following Cryopreservation.  Cryobiology 30:386-395.

Harvell, J.D., Owicki, J.C., & Maibach, H.I. (1994) The Silicon Microphysiometer:  Use in Ocular and Cutaneous Toxicity Testing.  Alt. Meth. Toxicol. 10:79-82.

Baxter, G.T., Young, M.-L., Miller, D.L., & Owicki, J.C. (1994) Using Microphysiometry to Study the Pharmacology of Exogenously Expressed m1 and m3 Muscarinic Receptors.  Life Sciences 55:573-583.

Thibodeau, A., Kuo, R.C., Crothers, J.M., Jr., Yao, X., Owicki, J.C., & Forte, J.G. (1994) Direct Measurement of Extracellular Proton Flux from Isolated Gastric Glands.  Am. J. Physiol. 267: C1473-C1482.

Salon, J.A., & Owicki, J.C. (1995) Real-Time Measurements of Receptor Activity:  Applications of Microphysiometric Techniques to Receptor Biology.   In: Methods in Neuroscience:  Receptor Molecular Biology (Ed. Sealfon, Academic, NY), vol. 25, pp. 201-224, invited chapter.

 

 

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